Design and synthesis of biotinylated inositol phosphates relevant to the biotin-avidin techniques.
نویسندگان
چکیده
Six bifunctional molecules containing biotin and various inositol phosphates were synthesized. These compounds were designed on the basis of X-ray structures of the complexes of D-myo-inositol 1,4,5-triphosphates (IP(3)) and phospholipase C delta pleckstrin homology domain (PLCdelta PH) considering the application to the biotin-avidin techniques. The building blocks of the inositol moiety were synthesized starting with optically resolved myo-inositol derivatives and assembled to the biotin linker through a phosphate linkage.
منابع مشابه
Purification of Saccharomyces cerevisiae eIF4E/eIF4G/Pab1p Complex with Capped mRNA
Protein synthesis is one of the most complex cellular processes, involving numerous translation components that interact in multiple sequential steps. The most complex stage in protein synthesis is the initiation process. The basal set of factors required for translation initiation has been determined, and biochemical, genetic, and structural studies are now beginning to reveal details of their...
متن کاملAggregation of biotinylated polymeric microspheres induced by interaction with avidin
Monodisperse biotinylated poly(styrene-co-N-acryloxysuccinimide) microspheres were synthesized in aqueous solutions with a two-step method. Upon the addition of avidin solutions of different concentrations in phosphate buffer into the dispersed biotinylated microspheres, the microspheres aggregated rapidly due to the high binding affinity between biotin and avidin. The hydrodynamic diameter of ...
متن کاملSynthesis of an amplifiable reporter RNA for bioassays.
The replacement of reporter groups, such as fluorescent molecules or enzymes, by an amplifiable reporter should lead to bioassays of greatly increased sensitivity, since a very large number of copies of the reporter can be accumulated in a short time. Midivariant RNA is an appropriate reporter, since it is autocatalytically replicated by Q beta RNA polymerase in vitro. This RNA can be amplified...
متن کاملSection 7.6 - Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices
The high affinity of avidin for biotin was first exploited in histochemical applications in the mid-1970s.1,2 This egg-white protein and its bacterial counterpart, streptavidin, have since become standard reagents for diverse detection schemes.3,4 In their simplest form, such methods entail applying a biotinylated probe to the sample and then detecting the bound probe with a labeled avidin or s...
متن کاملThermal-induced Immuno-nephelometry Using Gold Nanoparticles Conjugated with a Thermoresponsive Polymer for the Detection of Avidin.
Thermoresponsive immunonephelometry was achieved with biotinylated poly(acrylate) and thermoresponsive gold nanocomposites composed of 13-nm gold nanoparticles and thermoresponsive polymers containing triethylenetetramine and biotin groups. The avidin-biotin interaction was used to model an immunoreaction in order to demonstrate thermoresponsive immunonephelometry. In the absence of avidin, pos...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 6 10 شماره
صفحات -
تاریخ انتشار 2008